CDC26 Overexpression Lysate

Images

 
Western Blot: CDC26 Overexpression Lysate (Adult Normal) [NBL1-08997] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC26.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CDC26 Overexpression Lysate Summary

Description

CDC26 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC209719

Accession#: NM_139286

Protein Tag: C-MYC/DDK
Gene
CDC26

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
9.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CDC26 Overexpression Lysate

  • ANAPC12chromosome 9 open reading frame 17
  • Anaphase-promoting complex subunit 12
  • anaphase-promoting complex subunit CDC26
  • APC12anaphase promoting complex subunit 12
  • C9orf17
  • CDC26 subunit of anaphase promoting complex
  • cell division cycle 26 homolog (S. cerevisiae)
  • cell division cycle 26
  • Cell division cycle protein 26 homolog

Background

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc26, a component of cell cycle anaphase-promoting complex (APC). APC is composed of a group of highly conserved proteins and functions as a cell cycle-regulated ubiquitin-protein ligase. APC thus is responsible for the cell cycle regulated proteolysis of various proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CDC26 Lysate (NBL1-08997) (0)

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Video Protocols

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Bioinformatics

Gene Symbol CDC26