Cdc23 Antibody (SD08-60)

Western Blot: Cdc23 Antibody (SD08-60) [NBP2-67818] - Analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (SD08-60) [NBP2-67818] - ICC staining Cdc23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (SD08-60) [NBP2-67818] - ICC staining Cdc23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Product Discontinued

Cdc23 Antibody (SD08-60) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cdc23 aa 521-570 / 597. (SwissProt: Q9UJX2 Human; SwissProt: Q8BGZ4 Mouse; Unigene: 139314 Rat)
• Localization: Cytosol.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC23
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cdc23 Antibody (SD08-60)

• ANAPC8anaphase promoting complex subunit 8
• Anaphase-promoting complex subunit 8
• APC8CDC23 (cell division cycle 23, yeast, homolog)
• cell division cycle 23 homolog (S. cerevisiae)
• cell division cycle protein 23 homolog
• CUT23
• Cyclosome subunit 8

Background

CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC23