CDC16 Overexpression Lysate (Native)

Images

 
Western Blot: CDC16 Overexpression Lysate (Adult Normal) [NBL1-08989] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDC16.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CDC16 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205639 and based on accession number NM_003903. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cell division cycle 16 homolog (S. cerevisiae) (CDC16), transcript variant 1, mRNA.
Gene
CDC16

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
71.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CDC16

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CDC16 Overexpression Lysate (Native)

  • ANAPC6subunit 6
  • CDC16 (cell division cycle 16, S. cerevisiae, homolog)
  • CDC16 cell division cycle 16 homolog (S. cerevisiae)
  • CDC16 homolog
  • CDC16Hs
  • cell division cycle 16 homolog (S. cerevisiae)
  • cell division cycle protein 16 homolog
  • Cyclosome subunit 6

Background

This gene encodes a component protein of the APC complex, which is composed of eight proteins and functions as a protein ubiquitin ligase. The APC complex is a cyclin degradation system that governs exit from mitosis. Each component protein of the APC complex is highly conserved among eukaryotic organisms. This protein and two other APC complex proteins, CDC23 and CDC27, contain a tetratricopeptide repeat (TPR), a protein domain that may be involved in protein-protein interaction. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript Variant: This variant represents the longer transcript. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CDC16 Lysate (NBL1-08989) (0)

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Video Protocols

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Bioinformatics

Gene Symbol CDC16