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CDC16 Antibody (SD085-8)

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Western Blot: CDC16 Antibody (SD085-8) [NBP2-67820] - Analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
Western Blot: CDC16 Antibody (SD085-8) [NBP2-67820] - Analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: CDC16 Antibody (SD085-8) [NBP2-67820] - Staining APC6 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: CDC16 Antibody (SD085-8) [NBP2-67820] - Staining APC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: CDC16 Antibody (SD085-8) [NBP2-67820] - Staining APC6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
SD085-8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CDC16 Antibody (SD085-8) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant Protein (SwissProt: Q13042 Human ;SwissProt: Q8R349 Mouse ;Unigene: 66098 Rat)
Localization
Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDC16
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CDC16 Antibody (SD085-8)

  • ANAPC6subunit 6
  • CDC16 (cell division cycle 16, S. cerevisiae, homolog)
  • CDC16 cell division cycle 16 homolog (S. cerevisiae)
  • CDC16 homolog
  • CDC16Hs
  • cell division cycle 16 homolog (S. cerevisiae)
  • cell division cycle protein 16 homolog
  • Cyclosome subunit 6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Cdc27 Antibody [NBP1-89095] - Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: Cdc27 Antibody [NBP1-89095] - Staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells.
Immunocytochemistry/Immunofluorescence: Cdc27 Antibody [NBP1-89095] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
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Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human kidney tissue using anti-Protein C antibody. Counter stained with hematoxylin.
Species: Hu
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Free
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Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: APC Antibody [NB100-91662] - An intracellular stain was performed on HeLa cells with APC Antibody NB100-91662AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - Paraffin-embedded human colon carcinoma tissue.
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
USP6 Antibody
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Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunocytochemistry/Immunofluorescence: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: USP6 Antibody [NBP2-76350] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Species: Hu
Applications: Flow, ICC/IF, PEP-ELISA
Cdc23 Antibody
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Western Blot: Cdc23 Antibody (SD08-60) [NBP2-67818] - Analysis of Cdc23 on Hela cells lysates using anti-Cdc23 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (SD08-60) [NBP2-67818] - ICC staining Cdc23 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: Cdc23 Antibody (SD08-60) [NBP2-67818] - ICC staining Cdc23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
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Western Blot: TBC1D1 Antibody [NBP1-54654] - Antibody Titration: 1 ug/ml Human heart.
Western Blot: TBC1D1 Antibody [NBP1-54654] - Sample Tissue: Human ACHN Antibody Dilution: 1.0 ug/ml
Immunohistochemistry-Paraffin: TBC1D1 Antibody [NBP1-54654] - Human Kidney tissue, 5 ug/ml.
Species: Hu
Applications: IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: TPR Antibody [NB100-2866] - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue). Image submitted by a verified customer review.
Knockdown Validated: TPR Antibody [NB100-2866] - Lane 1 contians wildtype MDA-MB-231 cell lysate nuclear extract. Lanes 2, 3 and 4 contain the TPR knocked down MDA-MB-231 cell lysate nuclear extract. The lowered intensity of the bands in knocked down cell lines compared to wildtype supports antibody specificity to intended target protein. Image was submitted by customer review.
Western Blot: TPR Antibody [NB100-2866] - Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TPR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
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Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human stomach, upper shows moderate nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: CDC7 Antibody [NBP2-32708] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
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Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.
Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.
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Western Blot: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBC1D4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBC1D4.
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Hela cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: TBC1D4 Antibody (OTI5E6) - Azide and BSA Free [NBP2-74462] - Analysis of Jurkat cells, using anti-TBC1D4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Flow Cytometry: Apc1 Antibody [NBP1-77375] - APC1 expression in human lymphocytes. Image from verified customer review.
Western Blot: Apc1 Antibody [NBP1-77375] - analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 ug/ml.
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Immunohistochemistry-Paraffin: SLC25A24 Antibody [NBP2-57669] - Staining of human small intestine shows high expression.
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Western Blot: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPI64 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TBC1D10A Antibody [NBP2-36551] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated EPI64, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds
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Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
CDC26 Antibody
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Immunocytochemistry/Immunofluorescence: CDC26 Antibody [NBP1-33112] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Western Blot: CDC26 Antibody [NBP1-33112] - Sample (30 ug of whole cell lysate) A: H1299 15% SDS PAGE; antibody diluted at 1:1000.
Species: Hu
Applications: ICC/IF, WB
Apc10 Antibody
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ELISA: Apc10 Antibody (8F1D10) [NBP2-61889] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow Cytometry: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: Apc10 Antibody (8F1D10) [NBP2-61889] - Analysis of Hela cells using ANAPC10 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues . Corresponding CDK1 RNA-seq data are presented for the same tissues.
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in human cell line A-431.
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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Cyclin B1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of U2OS human osteosarcoma cell line, K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin B1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyclin B1 at approximately 67 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse Cyclin B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6000) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(4 Publications)
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Applications: IHC, Simple Western, WB

Publications for CDC16 Antibody (NBP2-67820) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDC16