CDC16 Antibody (SD085-8)

Western Blot: CDC16 Antibody (SD085-8) [NBP2-67820] - Analysis of APC6 on Hela cells lysates using anti-APC6 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: CDC16 Antibody (SD085-8) [NBP2-67820] - Staining APC6 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC16 Antibody (SD085-8) [NBP2-67820] - Staining APC6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC16 Antibody (SD085-8) [NBP2-67820] - Staining APC6 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

CDC16 Antibody (SD085-8) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CDC16 aa 181-247 / 620. (SwissProt: Q13042 Human; SwissProt: Q8R349 Mouse; Unigene: 66098 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC16
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC16 Antibody (SD085-8)

• ANAPC6subunit 6
• CDC16 (cell division cycle 16, S. cerevisiae, homolog)
• CDC16 cell division cycle 16 homolog (S. cerevisiae)
• CDC16 homolog
• CDC16Hs
• cell division cycle 16 homolog (S. cerevisiae)
• cell division cycle protein 16 homolog
• Cyclosome subunit 6

Background

CDC15/APC6 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. CDC15/APC6 amplification is associated with hepatocellular carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC16