CD74 Antibody (JF40-10)

Images

 
Western Blot: CD74 Antibody (JF40-10) [NBP2-66762] - Analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: CD74 Antibody (JF40-10) [NBP2-66762] - Staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: CD74 Antibody (JF40-10) [NBP2-66762] - Analysis of paraffin-embedded human tonsil tissue using anti-CD74 antibody. Counter stained with hematoxylin.
Flow Cytometry: CD74 Antibody (JF40-10) [NBP2-66762] - Analysis of Jurkat cells with CD74 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Immunocytochemistry/ Immunofluorescence: CD74 Antibody (JF40-10) [NBP2-66762] - Staining CD74 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunocytochemistry/ Immunofluorescence: CD74 Antibody (JF40-10) [NBP2-66762] - Staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunohistochemistry-Paraffin: CD74 Antibody (JF40-10) [NBP2-66762] - Analysis of paraffin-embedded human spleen tissue using anti-CD74 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
JF40-10
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CD74 Antibody (JF40-10) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Human CD74 aa 1-200
Localization
Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus, Endosome, Lysosome.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD74
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for CD74 Antibody (JF40-10)

  • CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
  • CD74 antigen
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • CD74
  • CLIP
  • DHLAG
  • DHLAGgamma chain of class II antigens
  • HLA class II histocompatibility antigen gamma chain
  • HLADG
  • HLA-DR antigens-associated invariant chain
  • HLA-DR-gamma
  • Ia antigen-associated invariant chain
  • Ia-associated invariant chain
  • Ia-gamma
  • Ii
  • INVG34
  • MHC HLA-DR gamma chain
  • p33

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD74 Antibody (NBP2-66762) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for CD74 Antibody (NBP2-66762)

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Research Areas for CD74 Antibody (NBP2-66762)

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Blogs on CD74.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

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Bioinformatics

Gene Symbol CD74