CD59 Antibody (MACIF/629) [DyLight 405]

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
• Clone: MACIF/629
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 405

CD59 Antibody (MACIF/629) [DyLight 405] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Human K562 tumor cells
• Localization: Cell Surface
• Specificity: Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This monoclonal antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD59
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-47820. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD59 Antibody (MACIF/629) [DyLight 405]

• 16.3A5
• 1F5 antigen
• 1F5
• 20 kDa homologous restriction factor
• CD59 antigen
• CD59 antigen, complement regulatory protein
• CD59 glycoprotein
• CD59 molecule, complement regulatory protein
• CD59
• EJ16
• EJ30
• EJ30, EL32 and G344)
• EL32
• FLJ38134
• FLJ92039
• G344
• HRF20
• HRF-20
• human leukocyte antigen MIC11
• Ly-6-like protein
• lymphocytic antigen CD59/MEM43
• MACIF
• MAC-inhibitory protein
• MAC-IP
• MEM43 antigen
• MEM43
• membrane attack complex (MAC) inhibition factor
• Membrane attack complex inhibition factor
• Membrane inhibitor of reactive lysis
• MGC2354
• MIC11
• MIC11MSK21
• MIN1
• MIN2
• MIN3
• MIRL
• p18-20
• Protectin
• surface anitgen recognized by monoclonal 16.3A5
• T cell-activating protein

Background

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD59