CD39L4/ENTPD5 Antibody

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human CD39L4/ENTPD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5297) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CD39L4/ENTPD5 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD39L4/ENTPD5 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD39L4/ENTPD5
  Val21-His428
  Accession # O75356
• Specificity: Detects human CD39L4/ENTPD5 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ENTPD5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39L4/ENTPD5 Antibody

• CD39 antigen-like 4
• CD39L4
• CD39L4NTPDase 5
• CD39-like 4
• EC 3.6.1
• EC 3.6.1.42
• EC 3.6.1.6
• ectonucleoside triphosphate diphosphohydrolase 5
• ENTPD5
• ER-UDPase
• GDPase ENTPD5
• Guanosine-diphosphatase ENTPD5
• MGC163357
• MGC163359
• NTPDase 5
• NTPDase-5
• Nucleoside diphosphatase
• Pcph proto-oncogene protein
• PCPH
• proto-oncogene CPH
• UDPase ENTPD5
• Uridine-diphosphatase ENTPD5

Background

CD39L4 (also ER-UDPase, PCPH and Ectonucleoside triphosphate diphosphohydrolase 5/eNTPDase5) is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation-dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa identity with mouse CD39L4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENTPD5
• Uniprot:
  • O75356(Human)