CD39/ENTPD1 Antibody (498409)

• Reactivity: Hu
• Applications: WB
• Clone: 498409
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD39/ENTPD1 Antibody (498409) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD39
  Thr38-Val478
  Accession # P49961
• Specificity: Detects human CD39/ENTPD1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD39 or recombinant human CD39L2, 3, or 4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENTPD1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD39/ENTPD1 Antibody (498409)

• ADPase
• ATPDase
• ATP-diphosphatase
• CD39 antigen
• CD39
• CD39EC 3.6.1.5
• DKFZp686D194
• DKFZp686I093
• EC 3.6.1
• ecto-apyrase
• Ecto-ATP diphosphohydrolase 1
• Ecto-ATPase 1
• Ecto-ATPDase 1
• ectonucleoside triphosphate diphosphohydrolase 1
• ENTPD1
• FLJ40921
• FLJ40959
• Lymphoid cell activation antigen
• NTPD1
• NTPDase 1
• NTPDase-1

Background

Ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1), also called NTPDase-1 and designated CD39, is an integral membrane protein with an extracellular active site. ENTPD1 was originally described as a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). ENTPD1 hydrolyzes the beta - and gamma phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, ENTPD1 plays a role in the regulation of purinergic signaling (4). ENTPD1 is involved in the processes of thromboregulation and vascular inflammation (5). The administration of soluble ENTPD1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6). The amino acid sequence of human ENTPD1 shares 77% and 75% identity with that of mouse and rat ENTPD1, respectively.

1. Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
2. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
3. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
4. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
5. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
6. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENTPD1
• Uniprot:
  • P49961()