CD300a/LMIR1 Antibody (172224) [Unconjugated]

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 172224
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD300a/LMIR1 Antibody (172224) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1
  Leu28-Arg183
  Accession # BAC80268
• Specificity: Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, approximately 2% cross-reactivity with recombinant mouse (rm) LMIR2 is observed and no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, or rmLMIR3, 4, or 5 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD300A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD300a/LMIR1 Antibody (172224) [Unconjugated]

• CD300a antigenCMRF35H9
• CD300a molecule
• CD300a
• CLM8
• CLM-8
• CMRF35H leukocyte immunoglobulin-like receptor
• CMRF-35H
• CMRF35-H
• CMRF35-H9
• CMRF-35-H9CD300 antigen-like family member A
• CMRF35HIRp60
• CMRF35-like molecule 8
• IGSF12
• IGSF12NK inhibitory receptor
• Immunoglobulin superfamily member 12
• Inhibitory receptor protein 60
• IRC1
• IRC1/IRC2
• IRC2
• IRp60
• leukocyte membrane antigen
• LMIR1
• MAIR-I
• Mcipr1
• NKRL
• Pigr4

Background

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.
3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.
4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.
5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.
6. Munitz, A. et al. (2006) Blood 107:1996.
7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.
8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.
9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.
10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD300a/LMIR1 Antibody (MAB1186)(3)

Publications using MAB1186

• L Karra, R Singh Gang, R Shamri, PG Puzzovio, S Cohen-Mor, BD Levy, F Levi-Schaf Leukocyte CD300a Contributes to the Resolution of Murine Allergic Inflammation J. Immunol., 2018-10-12;0(0):. 2018-10-12 [PMID: 30315138] (Engagement Assay, Flow Cytometry, Mouse)
• P Rozenberg, H Reichman, I Zab-Bar, M Itan, M Pasmanik-C, C Bouffi, U Qimron, I Bachelet, PC Fulkerson, ME Rothenberg, A Munitz CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production Sci Rep, 2017-07-19;7(1):5922. 2017-07-19 [PMID: 28725048] (Flow Cytometry, Mouse)
• Bachelet I, Munitz A, Mankutad D, Levi-Schaffer F Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J. Biol. Chem., 2006-07-10;281(37):27190-6. 2006-07-10 [PMID: 16831868] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CD300A
• Uniprot:
  • BAC80268()