Human CD3 gamma ELISA Kit (Colorimetric)

ELISA: Human CD3 gamma ELISA Kit (Colorimetric) [NBP2-75144] - Samples were spiked with high concentrations of Human CD3 gamma and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human CD3 gamma ELISA Kit (Colorimetric) [NBP2-75144] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human CD3 gamma ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CD3g in samples. No significant cross-reactivity or interference between Human CD3g and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.7%
• Intra-Assay: CV% < 3.78%
• Spike Recovery: 88-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CD3G

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CD3 gamma ELISA Kit (Colorimetric)

• CD3 gamma
• CD3g antigen
• CD3g antigen, gamma polypeptide (TiT3 complex)
• CD3g molecule, epsilon (CD3-TCR complex)
• CD3g molecule, gamma (CD3-TCR complex)
• CD3g
• CD3-GAMMA
• FLJ17620
• FLJ17664
• FLJ79544
• FLJ94613
• IMD17
• MGC138597
• T3G
• T-cell antigen receptor complex, gamma subunit of T3
• T-cell receptor T3 gamma chain
• T-cell surface glycoprotein CD3 gamma chain

Background

CD3G is encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for CD3 gamma ELISA Kit (NBP2-75144)(1)

Publication using NBP2-75144

• Takahashi H, KUhtreiber WM, Keefe RC et al. BCG vaccinations drive epigenetic changes to the human T cell receptor: Restored expression in type 1 diabetes Science advances 2022-11-18 [PMID: 36383663] (ELISA, Human)

FAQs for CD3 gamma ELISA Kit (NBP2-75144). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CD3G