CD160 Antibody (688327) [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
688327
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

CD160 Antibody (688327) [Alexa Fluor® 750] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD160
Ile27-Ser159
Accession # O95971
Specificity
Detects human CD160 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD160 is observed.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD160
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD160 Antibody (688327) [Alexa Fluor® 750]

  • BY55
  • BY55FLJ46513
  • CD160 antigenimmunoglobulin superfamily member
  • CD160 molecule
  • CD160
  • Natural killer cell receptor BY55
  • NK1
  • NK28

Background

CD160 (also BY55) is a 27 kDa member of the immunoglobulin superfamily of molecules.  It is expressed on select hematopoietic cell types, including CD56dim CD16+ cytotoxic NK cells, CD8+ CD28- effector T cells, δ/ gamma T cells, and restricted CD4+ T cells.  It is a receptor for HLA-C molecules, and its engagement induces CD160+ NK cells to both secrete IFN-gamma plus TNF-alpha and initiate a cytotoxic program.  Human CD160 was originally identified as a 155 amino acid (aa) proprotein (aa 27‑181).  It contains a 132 aa mature region (aa 27-159) and a C-terminal prosegment that is cleaved to create a GPI linkage. The mature region possesses one
V‑type Ig-like domain (aa 27-122).  CD160 is found as a soluble, disulfide-linked 80 kDa multimer (likely trimer) that is generated by proteolysis of the GPI-linked form. This 80 kDa form, plus others, are highly resistant to reduction. There is also a 100-110 kDa multimeric transmembrane (TM) form that is associated with activated NK cells. It contains a 55 aa substitution for Gly180-Leu181, and shows a 20 aa TM segment between aa 163-182.  The TM form appears to have a splice variant that lacks aa 25-133.  Over aa 27-159, human CD160 shares 62% aa identity with mouse CD160.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Allophycocyanin FAB6700A
Phycoerythrin FAB6700P

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Bioinformatics

Gene Symbol CD160
Uniprot