CCT5 Antibody (4E5-4B1)

Images

 
Western Blot: CCT5 Antibody (4E5-4B1) [H00022948-M01] - CCT5 monoclonal antibody (M01), clone 4E5-4B1 Analysis of CCT5 expression in Hela.
Immunocytochemistry/ Immunofluorescence: CCT5 Antibody (4E5-4B1) [H00022948-M01] - Analysis of monoclonal antibody to CCT5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CCT5 Antibody (4E5-4B1) [H00022948-M01] - Analysis of monoclonal antibody to CCT5 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
ELISA: CCT5 Antibody (4E5-4B1) [H00022948-M01] - Detection limit for recombinant GST tagged CCT5 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Clone
4E5-4B1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CCT5 Antibody (4E5-4B1) Summary

Immunogen
CCT5 (AAH06543, 1 a.a. ~ 541 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE
Specificity
CCT5 - chaperonin containing TCP1, subunit 5 (epsilon)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CCT5
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using H00022948-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CCT5 Antibody (4E5-4B1)

  • chaperonin containing TCP1, subunit 5 (epsilon)
  • epsilon subunit

Background

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CCT5 Antibody (H00022948-M01) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on CCT5

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Contact Information

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Bioinformatics

Gene Symbol CCT5
Entrez
Uniprot