Recombinant Human CCL4L1/LAG-1 Protein

SDS-Page: Human CCL4L1/LAG-1 Protein [NBP2-34929]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CCL4L1/LAG-1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL4L1/LAG-1 Source: E. coli

  Uniprot ID: Q8NHW4

  Amino Acid Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN

• Details of Functionality: CCL4L1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CCL4L2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CCL4L1/LAG-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute in sterile dH2O or aq buffer containing 0.1% BSA to a conc of 1mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Make further dilutions in buffered solution with NaCl conc >300mM.

Alternate Names for Recombinant Human CCL4L1/LAG-1 Protein

• AT744.2
• C-C motif chemokine 4-like
• CCL4L1
• CCL4Lsmall inducible cytokine A4-like
• chemokine (C-C motif) ligand 4-like 1
• LAG1
• LAG-1
• LAG-1/CCL4L1
• LAG1CCL4L2
• LAG-1chemokine (C-C motif) ligand 4-like
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• macrophage inflammatory protein-1b2
• MIP-1-beta
• Monocyte adherence-induced protein 5-alpha
• SCYA4L
• SCYA4L1
• SCYA4L2
• Small-inducible cytokine A4-like

Background

C-C motif chemokine 4-like, also known as MIP-1-beta, is encoded by one of several cytokine genes clustered on the q-arm of chromosome 17 and is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The human CCL4L1 cDNA encodes a 92 amino acid (a.a.) precursor with a 23 a.a. signal sequence and it shares greater than 98% a.a. sequence identity with CCL4 and CCL4L2. Recombinant Human CCL4L1 also shares 96% a.a. sequence identity with rhesus CCL4 and approximately 80 ~ 90% a.a. sequence identity with bovine, murine, rabbit, and rat CCL4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CCL4L2