Human CCL4L1/LAG-1 ELISA Kit (Colorimetric)

ELISA: Human CCL4L1/LAG-1 ELISA Kit (Colorimetric) [NBP2-75067] - Samples were spiked with high concentrations of Human CCL4L1/LAG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human CCL4L1/LAG-1 ELISA Kit (Colorimetric) [NBP2-75067] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human CCL4L1/LAG-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CCL4L1 in samples. No significant cross-reactivity or interference between Human CCL4L1 and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.22%
• Intra-Assay: CV% < 4.6%
• Spike Recovery: 88-112%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CCL4L2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CCL4L1/LAG-1 ELISA Kit (Colorimetric)

• AT744.2
• C-C motif chemokine 4-like
• CCL4L1
• CCL4Lsmall inducible cytokine A4-like
• chemokine (C-C motif) ligand 4-like 1
• LAG1
• LAG-1
• LAG1CCL4L2
• LAG-1chemokine (C-C motif) ligand 4-like
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• macrophage inflammatory protein-1b2
• MIP-1-beta
• Monocyte adherence-induced protein 5-alpha
• SCYA4L
• SCYA4L1
• SCYA4L2
• Small-inducible cytokine A4-like

Background

CCL4L1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals; most individuals have 1-5 copies in the diploid genome, although rare individuals do not contain this gene at all. The human genome reference assembly contains two copies of this gene. This record represents the more centromeric gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for CCL4L1/LAG-1 ELISA Kit (NBP2-75067)(1)

Publication using NBP2-75067

• Flerlage, T;Crawford, JC;Allen, EK;Severns, D;Tan, S;Surman, S;Ridout, G;Novak, T;Randolph, A;West, AN;Thomas, PG; Single cell transcriptomics identifies distinct profiles in pediatric acute respiratory distress syndrome Nature communications 2023-06-30 [PMID: 37391405] (ELISA)

FAQs for CCL4L1/LAG-1 ELISA Kit (NBP2-75067). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL4L2