![Western Blot: CCDC101 Antibody [NBP3-18637] - Figure 2. Western blot analysis using the antibody directed against CCDC101 Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against CCDC101 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 33 kDa) is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/CCDC101-Antibody-Western-Blot-NBP3-18637-img0001.jpg "Western Blot: CCDC101 Antibody [NBP3-18637] - Figure 2. Western blot analysis using the antibody directed against CCDC101 Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against CCDC101 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 33 kDa) is indicated on the right; the marker (in kDa) is shown on the left.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Novus Biologicals Rabbit CCDC101 Antibody (NBP3-18637) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Polyclonal antibody raised in rabbit against human CCDC101 (coiled-coil domain containing 101), using two KLH-conjugated synthetic peptides containing a sequence from the central and from the C-terminal part of the protein, respectively. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SGF29 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: CCDC101 Antibody [NBP3-18637] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human CCDC101 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,700.](http://images.novusbio.com/fullsize/CCDC101 Antibody-ELISA-NBP3-18637-img0002.jpg "ELISA: CCDC101 Antibody [NBP3-18637] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human CCDC101 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,700.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")