CBR3 Antibody

Western Blot: CBR3 Antibody [NB300-858] - (0.5ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CBR3 Antibody Summary

• Immunogen: Peptide with sequence C-QGQLVHDKVVQNW corresponding to C-Terminus according to NP_001227.1.
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CBR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in HEK293 lysates is reported in scientific literature (PMID: 20007405). An anonymous customer has reported positive results on human recombinant protein.
• Control:

  CBR3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBR3 Antibody

• carbonyl reductase (NADPH) 3
• carbonyl reductase (NADPH) 3, EC 1.1.1.18410EC 1.1.1
• carbonyl reductase [NADPH] 3
• carbonyl reductase 3
• EC 1.1.1.184
• hCBR3
• NADPH-dependent carbonyl reductase 3
• SDR21C2
• short chain dehydrogenase/reductase family 21C, member 2

Background

Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBR3 Antibody (NB300-858)(2)

Publications using NB300-858

• Bains OS, Karkling MJ, Lubieniecka JM et al. Naturally Occurring Variants of Human CBR3 Alter Anthracycline In Vitro Metabolism. J Pharmacol Exp Ther 2010-03-01 [PMID: 20007405]
• Watanabe K, Sugawara C, Ono A et al. Mapping of a novel human carbonyl reductase, CBR3, and ribosomal pseudogenes to human chromosome 21q22.2. Genomics 1998-08-15 [PMID: 9740676]

Bioinformatics

• Gene Symbol: CBR3
• Entrez:
  • 874(Human)
• Uniprot:
  • NP_001227.(Human)