Caspase-8 Antibody

Product Discontinued

Caspase-8 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 8. The immunogen region is: C-terminal.
• Specificity: Caspase 8 (Cleaved-Asp384) Antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Caspase-8 Antibody

• AIS
• androgen receptor
• CASP8
• Caspase8
• Caspase-8
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• Mch5
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Caspase-8 Antibody (NBP1-71399). (Showing 1 - 1 of 1 FAQ).

1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
   • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Bioinformatics

• Gene Symbol: CASP8
• Entrez:
  • 841(Human)
• Uniprot:
  • Q14790()