Caspase-14 Antibody

Product Discontinued

Caspase-14 Antibody Summary

• Immunogen: A 16 amino acid peptide from near the carboxy-terminus of human Caspase-14.
• Specificity: NB100-66071 detects an epitope within the C-terminal (CT) region of human caspase-14; a 27kD cysteine protease which is activated by zymogen processing during cellular differentiation. It is highly expressed by keratinocytes and trophoblast cells of the placenta, with lower expression in the choroid plexus, the pigmented layer of the retina, and hair follicles. Expression of the caspase-14 gene has recently been identified in epithelial tumours, including cervical and bladder carcinoma. The biological function of caspase-14 in tumour and normal tissue remains to be classified.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1 - 2ug/ml
• Application Notes: Western Blot In WB detects a band of approximately 27kDa in Jurkat cell lysates.

Reactivity Notes

Reacts with Rat, Mouse and Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate Buffered Saline
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Caspase-14 Antibody

• apoptosis-related cysteine protease
• CASP14
• CASP-14
• caspase 14, apoptosis-related cysteine peptidase
• caspase 14, apoptosis-related cysteine protease
• Caspase14
• Caspase-14
• EC 3.4.22.-
• MGC119078
• MGC119079
• MICE

Background

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP14
• Entrez:
  • 23581(Human)
  • 12365(Mouse)
  • 299587(Rat)