Caspase-14 Antibody

Product Discontinued

Caspase-14 Antibody Summary

• Immunogen: A 15 residue synthetic peptide derived from N terminal domain of Human Caspase 14.
• Localization: Cytoplasmic
• Specificity: This reacts with Caspase 14 in cell extracts.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP14
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:5000
• Application Notes: This antibody is useful for Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Caspase-14 Antibody

• apoptosis-related cysteine protease
• CASP14
• CASP-14
• caspase 14, apoptosis-related cysteine peptidase
• caspase 14, apoptosis-related cysteine protease
• Caspase14
• Caspase-14
• EC 3.4.22.-
• MGC119078
• MGC119079
• MICE

Background

Caspases are a family of cysteine proteases that play key roles in apoptosis. The precursor form of all caspaes is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunits, which associate into a2b2 tetramers to form the active enzyme. Caspase 14 also known as Mini ICE (MICE) is a member of the peptidase family C14 that has three potential isoforms. The pro form of Caspase 14 is approximately 29 kD. This caspase may be involved in the death receptor and granzyme B apoptotic pathways. Caspase 14 functions as downstream signal transducer of cell death that can be suppressed by retinoic acid. Caspase 14 becomes activated after granzyme B, Caspase 8, and Caspase 10 cleavage into large p18 and small p10 subunits. This caspase has been shown to associate with the large prodomain caspases, including Caspase 1, Caspase 2, Caspase 4, Caspase 8, and Caspase 10. Its high expression in embryonic tissues and limited expression in adult tissues suggests that it may have a role in ontogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP14
• Entrez:
  • 23581(Human)