Caspase-12 Antibody

Western Blot: Caspase-12 Antibody [NBP2-24618] - Analysis of Caspase-12 in human heart lysate in the 1) absence and 2) presence of immunizing peptide using Caspase-12 antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Product Discontinued

Caspase-12 Antibody Summary

• Immunogen: Made to amino acids between 100 and 150 of human Caspase-12.
• Predicted Species: Monkey (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP12
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in bovine and canine, and 83% homologous in mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for Caspase-12 Antibody

• Apoptosis Related Cysteine Protease
• CASP 12
• CASP12
• CASP12P1
• Caspase 12 pseudogene 1
• Caspase12
• Caspase-12
• UNQ9415

Background

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP12
• Uniprot:
  • Q6UXS9(Mouse)