Novus Biologicals products are now on bio-techne.com

Casein Kinase 2 beta Antibody [Unconjugated]

Images

 
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep ...read more
Casein Kinase 2 beta was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 2 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4380) at 10 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Casein Kinase 2 beta Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Casein Kinase 2 beta
Met1-His153
Accession # P67870
Specificity
Detects human, mouse and rat Casein Kinase 2 beta in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CSNK2B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Casein Kinase 2 beta Antibody [Unconjugated]

  • Casein Kinase 2 beta
  • casein kinase 2, beta polypeptide
  • Casein kinase II beta subunit
  • casein kinase II subunit beta
  • CK II beta
  • CK2B
  • CK2N
  • CSK2B
  • CSNK2B
  • G5A
  • MGC138222
  • MGC138224
  • Phosvitin
  • Protein G5a

Background

Casein kinase 2 (CK2) is a ubiquitous, tetrameric serine/threonine kinase comprised of two catalytic subunits (CK2 alpha and/or CK2 alpha ‘) and two identical regulatory subunits (CK2 beta ). CK2 has been implicated in numerous cellular processes, including signal transduction, transcription, translation, replication, and metabolic pathways. CK2 beta undergoes phosphorylation at Ser-2, Ser-3 and Ser-209. CK2 beta also plays dual roles in the regulation of CK2 acivity. The C-terminal domain is responsible for stable interactions with the catalytic subunit and increased catalytic activity following tetramer formation, while the N-terminal domain exerts negative regulation on the catalytic activity of CK2. CK2 is known to phosphorylate more than 300 different substrates. Phosphorylation of cell-cycle proteins such as p53, p34cdc2, p27KIP1, and MDM-2 account for the ability of CK2 to induce proliferation, while the phosphorylation of HS1, Bid, and Max account for its antiapoptotic role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14871
Species: Mu, Rt
Applications: ICC/IF, WB
NB120-2860
Species: Ba, Bv, Ch, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15669
Species: Hu, Mu, Rt
Applications: IHC-WhMt, IHC, WB
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
H00149420-Q01
Species: Hu
Applications: ELISA, AP, PA, WB
NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-81838
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-922
Species: Ch
Applications: ELISA, IHC, IP, Single-Cell Western, WB
NB600-717
Species: Bv, Ca, Ch, Fe, Gp, Hu, Ma, Mu, Po, Rb, Rt, Sh
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
NBP1-88188
Species: Hu
Applications: IHC, IHC-P
MAB7957
Species: Hu
Applications: ICC, IHC, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP1-85630
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-45184
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB100-56503
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-92546
Species: Hu, Mu, Rt
Applications: WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Casein Kinase 2 beta Antibody (AF4380) (0)

There are no publications for Casein Kinase 2 beta Antibody (AF4380).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Casein Kinase 2 beta Antibody (AF4380) (0)

There are no reviews for Casein Kinase 2 beta Antibody (AF4380). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Casein Kinase 2 beta Antibody (AF4380) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Casein Kinase 2 beta Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol CSNK2B
Uniprot