Casein Kinase 2 alpha Overexpression Lysate (Native)

Images

 
Western Blot: CKII alpha Lysate [NBL1-09536] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CSNK2A1

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Casein Kinase 2 alpha Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208789 and based on accession number NM_177560. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 3, mRNA.
Gene
CSNK2A1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Casein Kinase 2 alpha

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Casein Kinase 2 alpha Overexpression Lysate (Native)

  • Casein Kinase 2 alpha
  • casein kinase 2, alpha 1 polypeptide
  • casein kinase II alpha 1 subunit
  • CK II alpha
  • CK2 catalytic subunit alpha
  • CK2A1
  • CK2A1casein kinase II subunit alpha
  • CKII
  • CSNK2A1
  • EC 2.7.11
  • EC 2.7.11.1
  • protein kinase CK2

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR as well as the first coding exon compared to variant 1, resulting in an isoform (b) which is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Casein Kinase 2 alpha Lysate (NBL1-09536) (0)

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Bioinformatics

Gene Symbol CSNK2A1
Entrez