Casein Kinase 1 gamma Antibody (3F10)

Western Blot: Casein Kinase 1 gamma Antibody (3F10) [H00053944-M03] - Analysis of CSNK1G1 expression in transfected 293T cell line by CSNK1G1 monoclonal antibody (M03), clone 3F10. Lane 1: CSNK1G1 transfected lysatE (48.511 KDa). Lane 2: Non-transfected lysate.

Western Blot: Casein Kinase 1 gamma Antibody (3F10) [H00053944-M03] - Western blot analysis of CSNK1G1 over-expressed 293 cell line, cotransfected with CSNK1G1 Validated Chimera RNAi or non-transfected control. Blot probed with H00053944-M03. GAPDH(36.1 kDa) used as loading control.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Casein Kinase 1 gamma Antibody (3F10) Summary

• Immunogen: CSNK1G1 (AAH17236, 293 a.a. ~ 393 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKT
• Specificity: CSNK1G1 (3F10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSNK1G1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and RNAi Validation.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Casein Kinase 1 gamma Antibody (3F10)

• Casein Kinase 1 gamma
• casein kinase 1, gamma 1
• casein kinase I isoform gamma-1
• CKI-gamma 1
• CSNK1G1
• EC 2.7.11
• EC 2.7.11.1

Background

Casein kinase I is the most abundant serine/threonine kinase in eukaryotic cell extracts. Multiple isoforms of the enzyme exist. The gamma-1 isoform is involved in growth and morphogenesis of eukaryotic cells.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSNK1G1
• Entrez:
  • 53944(Human)
• OMIM:
  • 606274(Human)
• Uniprot:
  • AAH17236(Human)