Casein Kinase 1 delta Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NRK rat normal kidney cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Casein Kinase 1d at approximately 49 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Casein Kinase 1d was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human/Mouse/Rat Casein Kinase 1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4568) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Casein Kinase 1 delta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CK1δ
  Arg316-Arg415
  Accession # P48730
• Specificity: Detects endogenous human, mouse and rat CK1δ in Western blots. In Western blots, this antibody does not cross‑react with recombinant human CK1 alpha or rhCK1 gamma ; reactivity with CK1 epsilon is unknown.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CSNK1D
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Casein Kinase 1 delta Antibody [Unconjugated]

• Casein Kinase 1 delta
• casein kinase 1, delta
• casein kinase I isoform delta
• CKId
• CKI-delta
• CSNK1D
• EC 2.7.11
• EC 2.7.11.1
• HCKID

Background

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha , gamma ¹, gamma ², gamma ³ ,δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. CK1δ phosphorylates p53 as well as MDM2 and seems to play a principal role in coordinating the effects of these two key regulator proteins on cell growth and genome integrity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Casein Kinase 1 delta Antibody (AF4568)(1)

Publication using AF4568

• Neus Barranco, Virginia Plá, Daniel Alcolea, Irene Sánchez-Domínguez, Reiner Fischer-Colbrie, Isidro Ferrer et al. Dense core vesicle markers in CSF and cortical tissues of patients with Alzheimer’s disease Translational Neurodegeneration 2021-12-01 [PMID: 34565482]

Bioinformatics

• Gene Symbol: CSNK1D
• Uniprot:
  • P48730()