CARS Antibody (S01-7D4)

Western Blot: CARS Antibody (S01-7D4) [NBP3-19844] - Western blot detection of CARS in Hela, A549, HL-60, U2OS, C6 cell lysates using NBP3-19844 (1:1000 diluted). Predicted band size: 86kDa. Observed band size: 86kDa.

Immunohistochemistry-Paraffin: CARS Antibody (S01-7D4) [NBP3-19844] - Immunohistochemistry of CARS in paraffin-embedded Human breast cancer tissue using NBP3-19844 at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S01-7D4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

CARS Antibody (S01-7D4) Summary

• Description: Novus Biologicals Rabbit CARS Antibody (S01-7D4) (NBP3-19844) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human CARS (Uniprot # P49589)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CARS1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/20
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for CARS Antibody (S01-7D4)

• CARS1
• CysRS
• cysteine translase
• cysteine tRNA ligase 1, cytoplasmic
• Cysteine--tRNA ligase
• cysteinyl-tRNA synthetase
• cysteinyl-tRNA synthetase, cytoplasmic
• EC 6.1.1
• EC 6.1.1.16
• MGC:11246

Background

CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARS1