Carboxypeptidase A4/CPA4 Overexpression Lysate

Images

 
Western Blot: Carboxypeptidase A4 Overexpression Lysate (Adult Normal) [NBL1-09426] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Carboxypeptidase A4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Carboxypeptidase A4/CPA4 Overexpression Lysate Summary

Description
Transient overexpression lysate of carboxypeptidase A4 (CPA4), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC208575 and based on accession number NM_016352. The protein contains a C-MYC/DDK Tag.
Gene
CPA4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
45.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Carboxypeptidase A4/CPA4 Overexpression Lysate

  • Carboxypeptidase A3
  • Carboxypeptidase A4
  • CPA3EC 3.4.17
  • CPA4
  • EC 3.4.17.-
  • EC 3.4.17.1

Background

This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness. [provided by RefSeq]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Other Available Formats

NBL1-09426

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Bioinformatics

Gene Symbol CPA4