Carbonic Anhydrase VB/CA5B Antibody

Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VB/CA5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3176) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase VB/CA5B at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Carbonic Anhydrase VB/CA5B was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Carbonic Anhydrase VB/ CA5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3176) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase VB/CA5B Antibody Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase VB/CA5B
  Cys34-Pro317
  Accession # Q9Y2D0
• Specificity: Detects human Carbonic Anhydrase VB/CA5B in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CA-5A, rhCA-2, and rhCA-7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CA5B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase VB/CA5B Antibody

• CA5B
• Carbonate dehydratase VB
• carbonic anhydrase 5B, mitochondrial
• Carbonic Anhydrase VB
• carbonic anhydrase VB, mitochondrial
• carbonic dehydratase
• CAVB
• CA-VB
• EC 4.2.1.1
• MGC39962

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VB encoded by the CA5B gene is a mitochondrial protein. In comparison with another mitochondrial CA (CA5A), CA5B has different tissue distribution and chromosomal location (2, 3). The amino acid sequence of human CA5B (residues 34 to 317) is 94%, 93%, 92%, and 74% identical to that of canine, mouse, bovine/rat, and chicken.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
   
2. Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
3. Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA5B
• Uniprot:
  • Q9Y2D0()