CAPN14 Overexpression Lysate (Native)

Images

 
Western Blot: CAPN14 Overexpression Lysate (Adult Normal) [NBP2-09728] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CAPN14.

Product Details

Summary
Applications WB

Order Details

CAPN14 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001138594. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CAPN14, mRNA.
Gene
CAPN14

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
79.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CAPN14

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CAPN14 Overexpression Lysate (Native)

  • calpain 14
  • calpain14
  • calpain-14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30943
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50444
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NB600-836
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-919
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
NBP1-86328
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1034
Species: Mu, Rt
Applications: WB, Simple Western, IHC
NBP2-55057
Species: Hu
Applications: ICC/IF
NB120-3588
Species: Hu, Mu, Rt, Po, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
NBP2-14435
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-15675
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-31864
Species: Hu
Applications: IHC, IHC-P
NB600-844
Species: Hu, Mu, Rt, Ch, Rb
Applications: WB, IHC, IHC-Fr, IHC-P, IF
NBP2-57200
Species: Hu
Applications: ICC/IF
NBP2-09728
Applications: WB

Publications for CAPN14 Lysate (NBP2-09728) (0)

There are no publications for CAPN14 Lysate (NBP2-09728).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CAPN14 Lysate (NBP2-09728) (0)

There are no reviews for CAPN14 Lysate (NBP2-09728). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CAPN14 Lysate (NBP2-09728) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CAPN14 Overexpression Lysate (Native) Products

CAPN14 NBP2-09728

Related Products by Gene

Bioinformatics Tool for CAPN14 Lysate (NBP2-09728)

Discover related pathways, diseases and genes to CAPN14 Lysate (NBP2-09728). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CAPN14 Lysate (NBP2-09728)

Discover more about diseases related to CAPN14 Lysate (NBP2-09728).
 

Pathways for CAPN14 Lysate (NBP2-09728)

View related products by pathway.

PTMs for CAPN14 Lysate (NBP2-09728)

Learn more about PTMs related to CAPN14 Lysate (NBP2-09728).

Blogs on CAPN14

There are no specific blogs for CAPN14, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CAPN14 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CAPN14

Customers Who Bought This Also Bought