CaMKK alpha Antibody (1F6) - Azide and BSA Free

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - CAMKK1 monoclonal antibody (M01), clone 1F6 Analysis of CAMKK1 expression in Jurkat.

Immunohistochemistry-Paraffin: CaMKK alpha Antibody (1F6) [H00084254-M01] - Analysis of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1 ug/ml.

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - CAMKK1 over-expressed 293 cell line. Lane 2 cotransfected with CAMKK1 Validated Chimera RNAi or non-transfected control. Blot probed with H00084254-M01. GAPDH (36.1 kDa) used as loading control.

Western Blot: CaMKK alpha Antibody (1F6) [H00084254-M01] - Analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody (M01), clone 1F6.Lane 1: CAMKK1 transfected lysate(57.31 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: CaMKK alpha Antibody (1F6) [H00084254-M01] - Detection limit for recombinant GST tagged CAMKK1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, Mycoplasma
• Clone: 1F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CaMKK alpha Antibody (1F6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CaMKK alpha Antibody (1F6) - Azide and BSA Free (H00084254-M01) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CAMKK1 (AAH31647, 1 a.a. ~ 520 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLALQNQAQNIQLDSTNIAKPHSLLPSEQQDSGSTWAARSVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEGPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLV
• Specificity: CAMKK1 - calcium/calmodulin-dependent protein kinase kinase 1, alpha
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMKK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CaMKK alpha Antibody (1F6) - Azide and BSA Free

• calcium/calmodulin-dependent protein kinase kinase 1
• calcium/calmodulin-dependent protein kinase kinase 1, alpha
• Calcium/calmodulin-dependent protein kinase kinase alpha
• CaM-kinase IV kinase
• CaM-kinase kinase 1
• CaM-kinase kinase alpha
• CaM-KK 1
• CAMKK alpha protein
• CaMKK alpha
• CaM-KK alpha
• CaMKK1
• CAMKKACaMKK 1
• DKFZp761M0423
• EC 2.7.11
• EC 2.7.11.17
• MGC34095

Background

The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMKK1
• Entrez:
  • 84254(Human)
• Uniprot:
  • AAH31647(Human)