Recombinant Human CaMKI gamma Protein

Product Discontinued

Recombinant Human CaMKI gamma Protein Summary

• Description: Recombinant protein for Human CaMKI gamma
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_020439)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: CAMK1G
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 151 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human CaMKI gamma Protein

• calcium/calmodulin-dependent protein kinase IG
• calcium/calmodulin-dependent protein kinase type 1G
• CaM kinase I gamma
• CaM kinase IG
• CaMKI gamma
• CaM-KI gamma
• caMKIG
• CaMK-like CREB kinase III
• CLICKIII
• dJ272L16.1
• EC 2.7.11
• EC 2.7.11.17
• VWS1CLICK III

Background

CLICK-III/CaMKIG is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase (CaMK), an isoform of the CaMKI family with an extended C-terminal domain ending with CAAX motif (where AA is aliphatic acid). As expected from the similarity of its kinase domain with the other CaMKI isoforms, full activation of CLICK-III/CaMKIG required both Ca(2+)/CaM and phosphorylation by CaMKK. Ca(2+)/cAMP-response element-binding protein (CREB) was a good substrate for CLICK-III/CaMKIG, at least in vitro. Interestingly enough, CLICK-III/CaMKIG transcripts were most abundant in neurons, with the highest levels in limited nuclei such as the central nucleus of the amygdala (CeA) and the ventromedial hypothalamus. Consistent with the presence of the CAAX motif, CLICK-III/CaMKIG was found to be anchored to various membrane compartments, especially to Golgi and plasma membranes. Both point mutation in the CAAX motif and treatment with compactin, a HMG-CoA reductase inhibitor, disrupted such membrane localization, suggesting that membrane localization of CLICK-III/CaMKIG occurred in a prenylation-dependent way. These findings provide a novel mechanism by which neuronal CaMK activity could be targeted to specific membrane compartments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CAMK1G