CaM Kinase II delta Antibody

Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, K562 human chronic myelogenous leukemia cell line, MDA-MB-468 human breast cancer cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CaM Kinase IId Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CaM Kinase IId at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CaM Kinase IId was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using 15 µg/mL Sheep Anti-Human CaM Kinase IId Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4176) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CaM Kinase II delta Antibody Summary

• Immunogen: E. coli-derived recombinant human CaM Kinase IIδ
  Ala402-Lys478
  Accession # Q13557
• Specificity: Detects human CaM Kinase IIδ isoforms. No cross-reactivity with recombinant human CaM Kinase II alpha , CaM Kinase II beta , or CaM Kinase II gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CAMK2D
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CaM Kinase II delta Antibody

• calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
• calcium/calmodulin-dependent protein kinase II delta
• calcium/calmodulin-dependent protein kinase type II delta chain
• calcium/calmodulin-dependent protein kinase type II subunit delta
• CaM kinase II delta subunit
• CaM Kinase II delta
• CaM kinase II subunit delta
• CAMK2D
• CAMKD
• CaMK-II delta subunit
• CaMK-II subunit delta
• CaM-kinase II delta chain
• DKFZp686G23119
• DKFZp686I2288
• EC 2.7.11
• EC 2.7.11.17
• MGC44911

Background

Calcium/calmodulin-dependent protein kinase type II (CaMKII) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha , beta , gamma , and δ, and each can yield several isoforms through alternative splicing. Each CaMKII protein assembles into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous, and has been identified as the predominant isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2D
• Uniprot:
  • Q13557()