CaM Kinase II [p Thr286] Antibody (JE51-81)

Western Blot: CaM Kinase II [p Thr286] Antibody (JE51-81) [NBP3-32752] - Western blot analysis of CaM Kinase II on different lysates with Rabbit anti-CaM Kinase II antibody (NBP3-32752) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 54~73 kDa Observed band size: 50~60 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32752) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE51-81
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CaM Kinase II [p Thr286] Antibody (JE51-81) Summary

• Description: Novus Biologicals Rabbit CaM Kinase II [p Thr286] Antibody (JE51-81) (NBP3-32752) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr286 of human CaM Kinase II. (Uniprot: Q13554)
• Modification: p Thr286
• Localization: Synapse, Postsynaptic density, Cell projection, dendritic spine, dendrite.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAMK2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CaM Kinase II [p Thr286] Antibody (JE51-81)

• Calcium/calmodulin-dependent protein kinase;Calcium/calmodulin-dependent protein kinase type II subunit beta;CAM2;CAMK2;CAMK2B;CAMKB;CaMKIIbeta;MRD54
• CaM Kinase II
• CAMK2
• CAMK2B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2B