Mouse CaM Kinase II alpha ELISA Kit (Colorimetric)

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, Cell lysates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL
• Sensitivity: 0.117 ng/mL

Mouse CaM Kinase II alpha ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 0.312 - 20 ng/mL
• Sensitivity: 0.117 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12
• Intra-Assay: %CV < 10
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CAMK2A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CaM Kinase II alpha ELISA Kit (Colorimetric)

• calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• calcium/calmodulin-dependent protein kinase II alpha
• calcium/calmodulin-dependent protein kinase II alpha-B subunit
• CaM kinase II alpha subunit
• CaM Kinase II alpha
• CaM kinase II subunit alpha
• CAMK2A
• CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
• CaMK-II alpha subunit
• CaMK-II subunit alpha
• CaMKIINalpha
• CaM-kinase II alpha chain
• EC 2.7.11
• EC 2.7.11.17
• KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2A