CaM Kinase II alpha Antibody

Product Discontinued

CaM Kinase II alpha Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 260-310 of Human CaMKIIalpha.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMK2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of CaMK2-a (K292) pAb in extracts from A431 cells.
  Immunohistochemistry (IHC) analyzes of CaMK2-a (K292) pAb in paraffin-embedded human A431 tissue.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CaM Kinase II alpha Antibody

• calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• calcium/calmodulin-dependent protein kinase II alpha
• calcium/calmodulin-dependent protein kinase II alpha-B subunit
• CaM kinase II alpha subunit
• CaM Kinase II alpha
• CaM kinase II subunit alpha
• CAMK2A
• CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
• CaMK-II alpha subunit
• CaMK-II subunit alpha
• CaMKIINalpha
• CaM-kinase II alpha chain
• EC 2.7.11
• EC 2.7.11.17
• KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

The multifunctional CAMK2, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CAMK2 is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory. CAMK2B is the beta chain. There are seven named isoforms produced by alternative splicing. The variable region of the CAMK2B protein is encoded by at least 7 exons (V1 to V7). Alternative splicing within this region gives rise to CAMK2B isoforms.CAMK2G is the gamma chain. CAMK2G has six alternatively spliced variants that encode six different isoforms. Some of these variants have been identified in human tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaM Kinase II alpha Antibody (NBP1-20008)(3)

Publications using NBP1-20008

• Amar F. Cellular And Molecular Mechanism Of Action Of The Amyloid-Beta Oligomer Abeta Star 56. Thesis. 2016-01-01
• Amar F, Sherman MA, Rush T et al. The amyloid-B oligomer AB*56 induces specific alterations in neuronal signaling that lead to tau phosphorylation and aggregation. Sci Signal. 2017-05-09 [PMID: 28487416] (Human)
• Hall K, Yang S, Sauchanka, O et al. Behavioural deficits in transgenic mice expressing human truncated (1-120 amino acid) alpha-synuclein. Exp. Neurol. 2014-11-20 [PMID: 25450466]

Bioinformatics

• Gene Symbol: CAMK2A
• Entrez:
  • 815(Human)
  • 12322(Mouse)
  • 25400(Rat)