Calsequestrin 1 Antibody (VIIID12)

Western Blot: Calsequestrin 1 Antibody (VIIID12) [NB120-2824] - Analysis of canine skeletal muscle extract.

Immunocytochemistry/Immunofluorescence: Calsequestrin 1 Antibody (VIIID12) [NB120-2824] - Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calsequestrin at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody (VIIID12) [NB120-2824] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.

Immunocytochemistry/Immunofluorescence: Calsequestrin 1 Antibody (VIIID12) [NB120-2824] - Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calsequestrin at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: Calsequestrin 1 Antibody (VIIID12) [NB120-2824] - Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calsequestrin at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody (VIIID12) [NB120-2824] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.

Product Discontinued

Calsequestrin 1 Antibody (VIIID12) Summary

• Immunogen: Purified rabbit skeletal muscle sarcoplasmic reticulum.
• Localization: Isoform 1: Sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial Isoform 2: Smooth endoplasmic reticulum.
• Specificity: This recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASQ1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:20
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 63 kDa protein representing calsequestrin from canine skeletal muscle extracts. Higher molecular weight proteins are seen on the WB and are believed to be calsequestrin-like proteins found in the sarcoplasmic reticulum.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 15684334). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Calsequestrin 1 Antibody (VIIID12)

• calmitin
• calmitine
• calsequestrin 1 (fast-twitch, skeletal muscle)
• Calsequestrin, skeletal muscle isoform
• calsequestrin-1
• CASQ
• PDIB1

Background

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calsequestrin 1 Antibody (NB120-2824)(4)

Publications using NB120-2824

• Leatherbury L, Yu Q, Chatterjee B et al. A novel mouse model of X-linked cardiac hypertrophy. Am J Physiol Heart Circ Physiol 2008-04-18 [PMID: 18424640]
• Phimister AJ, Lango J, Lee EH, Ernst-Russell MA, Takeshima H, Ma J, Allen PD, Pessah IN. Conformation-dependent stability of junctophilin 1 (JP1) and ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J Biol Chem. 282(12):8667-77. 2007-03-23 [PMID: 17237236]
• Nissinen M, Kaisto T, Salmela P, Peltonen J, Metsikko K. Restricted distribution of mRNAs encoding a sarcoplasmic reticulum or transverse tubule protein in skeletal myofibers. J Histochem Cytochem;53(2):217-27. 2005-02-01 [PMID: 15684334]
• Semsarian C, Ahmad I, Giewat M, Georgakopoulos D, Schmitt JP, McConnell BK, Reiken S, Mende U, Marks AR, Kass DA, Seidman CE, Seidman JG. The L-type calcium channel inhibitor diltiazem prevents cardiomyopathy in a mouse model. J Clin Invest;109(8):1013-20. 2002-04-01 [PMID: 11956238]

Review for Calsequestrin 1 Antibody (NB120-2824) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 01/24/2018

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle cell lysate
• Species: Mouse
• Lot: RK243665

Bioinformatics

• Gene Symbol: CASQ1
• Entrez:
  • 844(Human)
  • 12372(Mouse)
  • 100009095(Rabbit)
• Uniprot:
  • P07221(Rabbit)