Calreticulin-2/CALR3 Antibody (4E3)

Western Blot: Calreticulin-2/CALR3 Antibody (4E3) [H00125972-M01] - Analysis of CALR3 expression in transfected 293T cell line by CALR3 monoclonal antibody (M01), clone 4E3.Lane 1: CALR3 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

Western Blot: Calreticulin-2/CALR3 Antibody (4E3) [H00125972-M01] - Analysis of CALR3 over-expressed 293 cell line, cotransfected with CALR3 Validated Chimera RNAi ( Cat # H00125972-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CALR3 monoclonal antibody (M01), clone 4E3 (Cat # H00125972-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: Calreticulin-2/CALR3 Antibody (4E3) [H00125972-M01] - Detection limit for recombinant GST tagged CALR3 is approximately 1ng/ml as a capture antibody.

Product Discontinued

Calreticulin-2/CALR3 Antibody (4E3) Summary

• Immunogen: CALR3 (AAH14595, 21 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VYFQEEFLDGEHWRNRWLQSTNDSRFGHFRLSSGKFYGHKEKDKGLQTTQNGRFYAISARFKPFSNKGKTLVIQYTVKHEQKMDCGGGYIKVFPADIDQKNLNGKSQYYIMFGPDICGFDIKKVHVILHFKNKYHENKKLIRCKVDGFTHLYTLILRPDLSYDVKIDGQSIESGSIEYDWNLTSLKKETSPAESKDWEQTKDNKAQDWEKHFLDASTSKQSDWNGDLDGDWPAPMLQKPPYQDGLKPEGIHKDVWLHRKMKNTDYLTQYDLSEFENIGAIGLELWQVRSGTIFDNFLITDDEEYADNFGKATWGETKGPEREMDAIQAKEEMKKAREEEEEELLSGKINRHEHYFNQFHRRNEL
• Specificity: CALR3 (4E3)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALR3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Calreticulin-2/CALR3 Antibody (4E3)

• CALR3
• calreticulin 2
• calreticulin 3
• Calreticulin2
• Calreticulin-2
• calreticulin-3
• cancer/testis antigen 93
• Crt2
• CRT2calreticulin-2
• CT93
• FLJ25355
• MGC26577

Background

Calreticulins, such as CALR3, are Ca(2+)-binding chaperones localized mainly in the endoplasmic/sarcoplasmic reticulum Persson et al. (2002) [PubMed 12384296].[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALR3
• Entrez:
  • 125972(Human)
• Uniprot:
  • AAH14595(Human)