Calpain 9 Antibody

Western Blot: Calpain 9 Antibody [NBP2-14896] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE, antibody diluted at 1:3000.

Immunocytochemistry/Immunofluorescence: Calpain 9 Antibody [NBP2-14896] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calpain 9 protein stained by Calpain 9 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Calpain 9 antibody [N1N3] detects Calpain 9 protein at cytoplasm by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calpain 9 protein stained by Calpain 9 antibody [N1N3] (NBP2-14896) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Calpain 9 Antibody Summary

• Description: Novus Biologicals Rabbit Calpain 9 Antibody (NBP2-14896) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Calpain 9. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPN9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Calpain 9 Antibody

• calpain 9 (nCL-4)
• calpain 9
• calpain-9
• Digestive tract-specific calpain
• EC 3.4.22
• EC 3.4.22.54
• GC36
• NCL4
• nCL-4EC 3.4.22.-
• New calpain 4
• novel calpain large subunit-4
• Protein CG36

Background

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CAPN9
• Uniprot:
  • O14815(Human)