Caldesmon/CALD1 Antibody (CALD 8)

Product Discontinued

Caldesmon/CALD1 Antibody (CALD 8) Summary

• Immunogen: Turkey gizzard caldesmon was used as the immunogen.
• Epitope: This antibody is directed against an epitope located on the calmodulin non-binding part of the molecule.
• Localization: Cytoplasmic
• Specificity: The localizes the high molecular weight form of caldesmon from pig stomach, chicken or turkey gizzard, and the low molecular weight form of caldesmon from chicken fibroblasts when used in an immunoblot technique.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALD1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Radioimmunodiffusion
  • Western Blot 1:100-1:2000
• Application Notes: DID: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. WB: Use at a dilution of 1/400 using a pig stomach caldesmon extract and immunoperoxidase labeling.By indirect immunofluorescence monoclonal anti-caldesmon stains frozen tissue sections from chicken, turkey, rabbit, guinea pig, and human tissue preparations.Caldesmon occurs in both a high molecular weight (120- 150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Chicken, Guinea pig, Pig, Rabbit and Turkey.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Raw ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Caldesmon/CALD1 Antibody (CALD 8)

• CAD
• CALD1
• caldesmon 1
• Caldesmon
• CDM
• CDMH-CAD
• HCAD
• LCAD
• L-CAD
• MGC21352
• NAG22

Background

Monoclonal anti-Caldesmon may be used for the study of the involvement of caldesmon in contraction, cell movement, shape change, exocytosis and endocytosis. Caldesmon is an actin interacting and calmodulin binding protein found in smooth muscle and other cell types. Caldesmon occurs in both a high molecular weight (120-150 kDa) and a low molecular weight (71-80 kDa) form, depending on the tissue in which it is located. Caldesmon plays a major role in the regulation of smooth muscle and non-muscle contractile events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALD1
• Entrez:
  • 800(Human)