Calcineurin B Antibody (212306)

Western blot shows lysates of MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Calcineurin B Monoclonal Antibody (Catalog # MAB1348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 212306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Calcineurin B Antibody (212306) Summary

• Immunogen: E. coli-derived recombinant human Calcineurin B
  Met1-Val170
  Accession # P63098
• Specificity: Detects endogenous human, mouse, and rat Calcineurin B.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP3R1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calcineurin B Antibody (212306)

• Calcineurin B
• calcineurin subunit B type 1
• CALNB1
• CNA2
• Cnb1
• MCIP1
• PP2B beta 1
• PPP3R1
• Protein phosphatase 2B regulatory subunit 1
• protein phosphatase 2B regulatory subunit B alpha
• protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I)
• protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I)
• protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
• Protein phosphatase 3 regulatory subunit B alpha isoform 1
• protein phosphatase 3, regulatory subunit B, alpha
• type I (19kDa)

Background

Background

Calcineurin, also called Protein Phosphatase 2B,

PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is

an enzyme that dephosphorylates serine and

threonine residues in proteins. It is a heterodimer of

a 59,000 dalton catalytic A subunit and a

1,900 dalton regulatory B subunit that is activated

by the binding of calcium ions and calmodulin (1).

Calcineurin is expressed in many tissues, but its

levels are highest in the brain, where it may play a

role in learning and memory (2). It has many

substrates, including NFAT, a transcription factor

that is activated by dephosphorylation (3).

Complexes of the immunosuppressants cyclosporin

and FK506 with immunophilin proteins such as

cyclophilin and FKBP12 are potent and specific

inhibitors of Calcineurin activity (4). Alterations in

Calcineurin activity are suspected to play a role in

cardiac hypertrophy (5) and graft versus host

disease in organ transplantation (6).

References:

1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.

2. Zeng H. et al. (2001) Cell 107:617.

3. Okamura, H. et al. (2000) Molecular Cell 6:539.

4. Liu, J. et al. (1992) Biochemistry 31:3896.

5. Molkentin, J.D. (2000) Circulation Research 87:731.

6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calcineurin B Antibody (MAB1348)(2)

Publications using MAB1348

• Mondal A, Appu AP, Sadhukhan T et al. Ppt1-deficiency dysregulates lysosomal Ca++ -homeostasis contributing to pathogenesis in a mouse model of CLN1 disease Journal of inherited metabolic disease 2022-02-12 [PMID: 35150145]
• Ying Zhu, Min Zhang, Fan Wang, Jing Sun, Jianda Lu, Ruiying Chen et al. Calcineurin B1 subunit in human peripheral blood mononuclear cells and its role in idiopathic membranous nephropathy Medicine (Baltimore) 2019-04-16 [PMID: 30985728]

Bioinformatics

• Gene Symbol: PPP3R1
• Uniprot:
  • P63098()