Calcineurin B Antibody (212306) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 212306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Calcineurin B Antibody (212306) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Calcineurin B
  Met1-Val170
  Accession # P63098
• Specificity: Detects endogenous human, mouse, and rat Calcineurin B.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Calcineurin B Antibody (212306) [Alexa Fluor® 488]

• Calcineurin B
• calcineurin subunit B type 1
• CALNB1
• CNA2
• Cnb1
• MCIP1
• PP2B beta 1
• PPP3R1
• Protein phosphatase 2B regulatory subunit 1
• protein phosphatase 2B regulatory subunit B alpha
• protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I)
• protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I)
• protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
• Protein phosphatase 3 regulatory subunit B alpha isoform 1
• protein phosphatase 3, regulatory subunit B, alpha
• type I (19kDa)

Background

Background

Calcineurin, also called Protein Phosphatase 2B,

PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is

an enzyme that dephosphorylates serine and

threonine residues in proteins. It is a heterodimer of

a 59,000 dalton catalytic A subunit and a

1,900 dalton regulatory B subunit that is activated

by the binding of calcium ions and calmodulin (1).

Calcineurin is expressed in many tissues, but its

levels are highest in the brain, where it may play a

role in learning and memory (2). It has many

substrates, including NFAT, a transcription factor

that is activated by dephosphorylation (3).

Complexes of the immunosuppressants cyclosporin

and FK506 with immunophilin proteins such as

cyclophilin and FKBP12 are potent and specific

inhibitors of Calcineurin activity (4). Alterations in

Calcineurin activity are suspected to play a role in

cardiac hypertrophy (5) and graft versus host

disease in organ transplantation (6).

References:

1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.

2. Zeng H. et al. (2001) Cell 107:617.

3. Okamura, H. et al. (2000) Molecular Cell 6:539.

4. Liu, J. et al. (1992) Biochemistry 31:3896.

5. Molkentin, J.D. (2000) Circulation Research 87:731.

6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P63098()