Calcineurin B Antibody (212306) [Alexa Fluor® 488]

• Applications: WB
• Clone: 212306
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Calcineurin B Antibody (212306) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Calcineurin B
  Met1-Val170
  Accession # P63098
• Specificity: Detects endogenous human, mouse, and rat Calcineurin B.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Background

Calcineurin, also called Protein Phosphatase 2B,

PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is

an enzyme that dephosphorylates serine and

threonine residues in proteins. It is a heterodimer of

a 59,000 dalton catalytic A subunit and a

1,900 dalton regulatory B subunit that is activated

by the binding of calcium ions and calmodulin (1).

Calcineurin is expressed in many tissues, but its

levels are highest in the brain, where it may play a

role in learning and memory (2). It has many

substrates, including NFAT, a transcription factor

that is activated by dephosphorylation (3).

Complexes of the immunosuppressants cyclosporin

and FK506 with immunophilin proteins such as

cyclophilin and FKBP12 are potent and specific

inhibitors of Calcineurin activity (4). Alterations in

Calcineurin activity are suspected to play a role in

cardiac hypertrophy (5) and graft versus host

disease in organ transplantation (6).

References:

1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.

2. Zeng H. et al. (2001) Cell 107:617.

3. Okamura, H. et al. (2000) Molecular Cell 6:539.

4. Liu, J. et al. (1992) Biochemistry 31:3896.

5. Molkentin, J.D. (2000) Circulation Research 87:731.

6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.