Calcineurin A Antibody (JA64-11)

Western Blot: Calcineurin A Antibody (JA64-11) [NBP2-67201] - Analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse brain tissue lysateLane 2: Mouse cerebellum tissue lysateLane 3: Rat brain tissue lysate

Flow Cytometry: Calcineurin A Antibody (JA64-11) [NBP2-67201] - Analysis of Hela cells with Calcineurin A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Calcineurin A Antibody (JA64-11) [NBP2-67201] - Analysis of Calcineurin A on different cells lysates using anti-Calcineurin A antibody at 1/500 dilution. Positive control: Line 1: Hela Line 2: 293 Line 3: A431

Western Blot: Calcineurin A Antibody (JA64-11) [NBP2-67201] - Analysis of Calcineurin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Hela cell lysateLane 2: 293 cell lysateLane 3: A431 cell lysate

Product Discontinued

Calcineurin A Antibody (JA64-11) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Calcineurin A aa 472-521 / 521. (SwissProt: Q08209 Human; SwissProt: P63328 Mouse; SwissProt: P63329 Rat)
• Localization: Cell membrane, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPP3CA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Calcineurin A Antibody (JA64-11)

• calcineurin A alpha
• Calcineurin A
• Calmodulin-dependent calcineurin A subunit alpha isoform
• CALN
• CALNA
• CALNAcatalytic subunit, alpha isoform
• CAM-PRP catalytic subunit
• CNA
• CNA1
• CNA1CALNA1
• EC 3.1.3.16
• PP2B
• PP3CA
• PPP2B
• PPP2BCCN1
• PPP3CA
• protein phosphatase 2B, catalytic subunit, alpha isoform
• protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform(calcineurin A alpha)
• protein phosphatase 3, catalytic subunit, alpha isozyme
• serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Background

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP3CA