CACNA2D1 Antibody

Western Blot: CACNA2D1 Antibody [NB100-60951] - Staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CACNA2D1 Antibody Summary

• Immunogen: Peptide with sequence C-QEIFNKYNKDKKVR corresponding to internal region according to NP_000713.2.
• Epitope: QEIFNKYNKDKKVR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CACNA2D1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 140 kDa band observed in human brain (cerebellum) lysates (calculated MW of 123 kDa band according to NP_000713.2). An anonymous customer has reported it not suitable on mouse and rat protein.
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACNA2D1 Antibody

• CACNA2
• CACNA2D1
• CACNL2A
• CACNL2Adihydropyridine-sensitive L-type, calcium channel alpha-2/delta subunit
• calcium channel, L type, alpha 2 polypeptide
• calcium channel, voltage-dependent, alpha 2/delta subunit 1
• CCHL2A
• MHS3
• voltage-dependent calcium channel subunit alpha-2/delta-1
• Voltage-gated calcium channel subunit alpha-2/delta-1

Background

This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CACNA2D1 Antibody (NB100-60951)(2)

Publications using NB100-60951

• Neely GG et al. A genome-wide Drosophila screen for heat nociception identifies a2d3 as ann evolutionarily conserved pain gene. Cell;143(4):628-38. 2010-11-12 [PMID: 21074052]
• Hullin R, Matthes J, von Vietinghoff S et al. Increased Expression of the Auxiliary beta(2)-subunit of Ventricular L-type Ca Channels Leads to Single-Channel Activity Characteristic of Heart Failure. PLoS ONE 2007-03-14 [PMID: 17356701]

Bioinformatics

• Gene Symbol: CACNA2D1
• Entrez:
  • 781(Human)
  • 12293(Mouse)
  • 25399(Rat)
• Uniprot:
  • P54289(Human)