Bub3 Antibody (JG38-81)

Images

 
Western Blot: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.Positive control:Lane 1: A549 Lane 2: HL-60Lane 3: A431 Lane 4: Rat ...read more
Immunocytochemistry/ Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded rat testis tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
Flow Cytometry: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of PC-3M cells with Bub3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). ...read more
Immunocytochemistry/ Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded human thyroid gland tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bub3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded mouse fallopian tissue using anti-Bub3 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
JG38-81
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Bub3 Antibody (JG38-81) Summary

Immunogen
Recombinant protein corresponding to the N-terminus of human Bub3.
Localization
Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BUB3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Bub3 Antibody (JG38-81)

  • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
  • BUB3 budding uninhibited by benzimidazoles 3 homolog
  • Bub3
  • BUB3L
  • budding uninhibited by benomyl
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • hBUB3
  • mitotic checkpoint component
  • mitotic checkpoint protein BUB3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BUB3