Bub3 Antibody (JG38-81)

Western Blot: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.Positive control:Lane 1: A549 Lane 2: HL-60Lane 3: A431 Lane 4: Rat colon

Immunocytochemistry/Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded rat testis tissue using anti-Bub3 antibody. Counter stained with hematoxylin.

Flow Cytometry: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of PC-3M cells with Bub3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Bub3 Antibody (JG38-81) [NBP2-75424] - Staining Bub3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded human thyroid gland tissue using anti-Bub3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bub3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Bub3 Antibody (JG38-81) [NBP2-75424] - Analysis of paraffin-embedded mouse fallopian tissue using anti-Bub3 antibody. Counter stained with hematoxylin.

Product Discontinued

Bub3 Antibody (JG38-81) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Bub3 aa 2-50 / 328. (SwissProt: O43684 Human; SwissProt: Q9WVA3 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BUB3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Bub3 Antibody (JG38-81)

• BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
• BUB3 budding uninhibited by benzimidazoles 3 homolog
• Bub3
• BUB3L
• budding uninhibited by benomyl
• budding uninhibited by benzimidazoles 3 homolog (yeast)
• hBUB3
• mitotic checkpoint component
• mitotic checkpoint protein BUB3

Background

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB3