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Bub3 Antibody (2H6) - BSA Free

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Western Blot: Bub3 Antibody (2H6) [NBP2-42680] - The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: Bub3 Antibody (2H6) [NBP2-42680] - The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BUB3 antibody (1:1000). Proteins were visualized using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
2H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-42680AF350
Alexa Fluor 405
NBP2-42680AF405
Alexa Fluor 488
NBP2-42680AF488
Alexa Fluor 532
NBP2-42680AF532
Alexa Fluor 594
NBP2-42680AF594
Alexa Fluor 647
NBP2-42680AF647
Alexa Fluor 700
NBP2-42680AF700
Alexa Fluor 750
NBP2-42680AF750
Biotin
NBP2-42680B
DyLight 350
NBP2-42680UV
DyLight 405
NBP2-42680V
DyLight 488
NBP2-42680G
DyLight 550
NBP2-42680R
DyLight 594
NBP2-42680DL594
DyLight 650
NBP2-42680C
DyLight 680
NBP2-42680FR
DyLight 755
NBP2-42680IR
FITC
NBP2-42680F
HRP
NBP2-42680H
Janelia Fluor 549
NBP2-42680JF549
Janelia Fluor 646
NBP2-42680JF646
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Bub3 Antibody (2H6) - BSA Free Summary

Immunogen
Recombinant human BUB3 (1-328aa) purified from E. coli
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BUB3
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot 1:1000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Bub3 Antibody (2H6) - BSA Free

  • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
  • BUB3 budding uninhibited by benzimidazoles 3 homolog
  • Bub3
  • BUB3L
  • budding uninhibited by benomyl
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • hBUB3
  • mitotic checkpoint component
  • mitotic checkpoint protein BUB3

Background

The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.
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Flow Cytometry: BubR1 Antibody (8G1) [NB100-353] - An intracellular stain was performed on HeLa cells with BubR1 Antibody (8G1)NB100-353C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.
Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.
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Western Blot: Mad Antibody [NBP2-85249] - Host: Rabbit. Target: MXD1. Positive control (+): Mouse small intestine (M-IN). Negative control (-): Mouse testis (M-TE). Antibody concentration: 1ug/ml
Western Blot: Mad Antibody [NBP2-85249] - Host: Mouse. Target Name: MXD1. Sample Tissue: Mouse Small Intestine. Antibody Dilution: 1ug/ml
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Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:10000
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat 2 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Western Blot: PTTG1 Antibody [NBP2-20287] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: PTTG1 Antibody [NBP2-20287] - Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTTG1 protein stained by Securin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunoprecipitation: PTTG1 Antibody [NBP2-20287] - Securin antibody immunoprecipitates Securin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of Securin protein by 2 µg of Securin antibody 12% SDS-PAGE The immunoprecipitated Securin protein was detected by Securin antibody diluted at 1:1000
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Bioinformatics

Gene Symbol BUB3
Uniprot