BST2 Antibody (44E9R) [Unconjugated]

Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-MouseBST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

BST-2/Tetherin was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 44E9R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

BST2 Antibody (44E9R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant extracellular domain of CD317
• Specificity: Detects mouse BST-2/Tetherin in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Bst2
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BST2 Antibody (44E9R) [Unconjugated]

• bone marrow stromal antigen 2
• bone marrow stromal cell antigen 2
• BST2
• BST-2
• CD317 antigen
• CD317
• HM1.24 Antigen
• NPC-A-7
• PDCA-1
• Tetherin

Background

BST-2, also known as Tetherin or PDCA1 and designated CD317, is a 30-35 kDa interferon-inducible protein that shows an unusual topology. The N-terminus is cytoplasmic, followed by a transmembrane segment, an extracellular loop, and a C-terminal GPI-linkage. BST-2 is expressed on bone marrow stromal cells and is upregulated in breast cancer and astrocytoma. It binds to ILT7 on plasmacytoid dendritic cells and inhibits proinflammatory TLR7 and TLR9 signaling. BST-2 inhibits the release of Kaposi sarcoma virus, HIV-1, and Lassa virus from infected cells, but this function is counteracted by viral proteins which directly bind and trigger the degradation of BST-2. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BST2 Antibody (MAB8660)(1)

Publication using MAB8660

• LC Dorman, PT Nguyen, CC Escoubas, ID Vainchtein, Y Xiao, PV Lidsky, H Nakajo, NJ Silva, C Lagares-Li, EY Wang, SE Taloma, B Cuevas, H Nakao-Inou, BM Rivera, B Schwer, C Condello, R Andino, TJ Nowakowski, AV Molofsky A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis bioRxiv : the preprint server for biology, 2023-03-20;0(0):. 2023-03-20 [PMID: 35233577] (IF, Mouse)

Bioinformatics

• Gene Symbol: Bst2