BRN3B/POU4F2 Antibody

Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] - (1ug/ml) staining of Human Cerebellum (A) + peptide (B), Mouse Brain (C) + peptide (D), and Rat Brain (E) + peptide (F) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BRN3B/POU4F2 Antibody Summary

• Immunogen: Peptide with sequence HGLPSHMGCMSDVD corresponding to internal region according to NP_004566.2.
• Specificity: This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU4F2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1-3 ug/ml
• Application Notes: Approx. 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 43.1kDa according to Human NP_004566.2). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Alternate Names for BRN3B/POU4F2 Antibody

• POU domain, class 4, transcription factor 2
• BRN3.2
• BRN3B
• Brn-3b
• POU class 4 homeobox 2

Background

POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POU4F2
• Entrez:
  • 5458(Human)