POU4F3 Antibody - BSA Free

Staining of human cell line U-2 OS shows localization to nucleoplasm.

ChIP-Exo-Seq composite graph for Anti-POU4F3 (NBP1-88349) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Mu, Rt
• Applications: ChIP, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POU4F3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POU4F3 Antibody - BSA Free (NBP1-88349) is a polyclonal antibody validated for use in IHC, ICC/IF and ChIP. Anti-POU4F3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDPR
• Predicted Species: Rat (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POU4F3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1-10µg per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry Reported in (PMID: 28402854)
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  POU4F3 Protein (NBP1-88349PEP)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:28402854).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for POU4F3 Antibody - BSA Free

• brain-3C
• Brain-specific homeobox/POU domain protein 3C
• BRN3.1
• Brn-3C
• BRN3Cbrn-3C
• DFNA15
• MGC138412
• POU class 4 homeobox 3
• POU domain class 4, transcription factor 3
• POU domain, class 4, transcription factor 3

Background

The POU4F3 gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU4F3 Antibody (NBP1-88349)(3)

Publications using NBP1-88349

• Wang G, Gu Y, Liu Z Deciphering the genetic interactions between Pou4f3, Gfi1 and Rbm24 in maintaining cochlear hair cell survival bioRxiv 2023-06-22 (IHC-P, Mouse)
• Guangqin Wang, Yunpeng Gu, Zhiyong Liu, Tanya T Whitfield, Kathryn Song Eng Cheah Deciphering the genetic interactions between Pou4f3, Gfi1, and Rbm24 in maintaining mouse cochlear hair cell survival eLife 2024-03-14 [PMID: 38483314]
• Walters B J, Coak E et al. In Vivo Interplay between p27Kip1, GATA3, ATOH1, and POU4F3 Converts Non-sensory Cells to Hair Cells in Adult Mice. Cell Rep 2017-11-04 [PMID: 28402854] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: POU4F3