BNIP2 Antibody (8C6) - Azide and BSA Free

Western Blot: BNIP2 Antibody (8C6) [H00000663-M02-100ug] - Analysis of BNIP2 expression in transfected 293T cell line by BNIP2 monoclonal antibody (M02), clone 8C6. Lane 1: BNIP2 transfected lysate (Predicted MW: 36 KDa). Lane 2: Non-transfected lysate.

Western Blot: BNIP2 Antibody (8C6) [H00000663-M02-100ug] - Analysis of BNIP2 expression in K-562.

ELISA: BNIP2 Antibody (8C6) [H00000663-M02-100ug] - Detection limit for recombinant GST tagged BNIP2 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 8C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

BNIP2 Antibody (8C6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse BNIP2 Antibody (8C6) - Azide and BSA Free (H00000663-M02-100ug) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: BNIP2 (AAH02461, 1 a.a. ~ 314 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGVELKEEWQDEDFPIPLPEDDSIEADILAITGPEDQPGSLEVNGNKVRKKLMAPDISLTLDPSDGSVLSDDLDESGEIDLDGLDTPSENSNEFEWEDDLPKPKTTEVIRKGSITEYTAAEEKEDGRRWRMFRIGEQDHRVDMKAIEPYKKVISHGGYYGDGLNAIVVFAVCFMPESSQPNYRYLMDNLFKYVIGTLELLVAENYMIVYLNGATTRRKMPSLGWLRKCYQQIDRRLRKNLKSLIIVHPSWFIRTLLAVTRPFISSKFSQKIRYVFNLAELAELVPMEYVGIPECIKQVDQELNGKQDEPKNEQ
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BNIP2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for BNIP2 Antibody (8C6) - Azide and BSA Free

• BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
• BCL2/adenovirus E1B 19kDa interacting protein 2
• BCL2/adenovirus E1B 19kD-interacting protein 2
• BNIP-2
• NIP2

Background

The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP2
• Entrez:
  • 663(Human)
• OMIM:
  • 603292(Human)
• Uniprot:
  • AAH02461(Human)