BNIP1 Antibody

Western Blot: BNIP1 Antibody [NBP2-89153] - Analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: BNIP1 Antibody [NBP2-89153] - Staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BNIP1 Antibody [NBP2-89153] - Analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: BNIP1 Antibody [NBP2-89153] - Staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: BNIP1 Antibody [NBP2-89153] - Analysis of paraffin-embedded human liver tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.

Product Discontinued

BNIP1 Antibody Summary

• Immunogen: Recombinant protein within Human BNIP1 aa 27-228 / 228. (SwissProt: Q12981 Human; SwissProt: Q8VHI8 Rat)
• Localization: Endoplasmic reticulum. Mitochondrion.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BNIP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:100
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BNIP1 Antibody

• BCL2/adenovirus E1B 19 kDa protein-interacting protein 1
• BCL2/adenovirus E1B 19kDa interacting protein 1
• BCL2/adenovirus E1B 19kD-interacting protein 1
• Nip1
• SEC20
• SEC20L
• Transformation-related gene 8 protein
• TRG-8
• vesicle transport protein SEC20

Background

BNIP1 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP1