BMI-1 Antibody - BSA Free

Western Blot: BMI-1 Antibody [NB600-487] - Analysis of a band 37 kDa corresponding to human Bmi1 (arrowhead). Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking in PBS containing 5% nonfat dry milk, the membrane was probed overnight at 4 C with the primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NB600-487] - Nuclear staining (green) of methanol fixed (100%, 5 min) HepG2 cells. The cells were blocked and permeabilized in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h prior to incubation with the primary antibody (1:200 dilution) overnight at +4C and detected with a 488nm fluorescent dye conjugated secondary Ab. Cell nuclei are stained with DAPI (blue) and plasma membranes are stained with WGA (red).

• Reactivity: Hu, Mu, Bv, Ch, Fe, Pm-Or
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free

BMI-1 Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human Bmi1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified BMI-1 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 252-264 of human BMI-1 protein. (Uniprot: P35226)
• Localization: Nuclear
• Predicted Species: Mouse (100%), Feline (100%), Bovine (100%), Orangutan (100%), Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BMI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:500-1:3000
• Application Notes: This affinity purified antibody has been tested for use in ELISA, immunofluorescence, and western blot. A 1:200 dilution is reported to be effective for Immunofluorescence against Methanol fixed cells. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 37 kDa in size corresponding to Bmi1 by western blotting in the appropriate cell lysate or extract.
• Control:

  BMI-1 Overexpression Lysate

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with BMI-1 protein from human, rat, orangutan, dog, chimpanzee, bovine and cat based on 100% homology to the immunogen sequence. This sequence shows significant homology to homologues from Xenopus and chicken sources. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BMI-1 Antibody - BSA Free

• B lymphoma Mo-MLV insertion region 1 homolog (mouse)
• B lymphoma Mo-MLV insertion region 1 homolog
• BMI1 polycomb ring finger oncogene
• BMI1
• BMI-1
• FLVI2/BMI1
• MGC12685
• murine leukemia viral (bmi-1) oncogene homolog
• PCGF4
• polycomb complex protein BMI-1
• polycomb group protein Bmi1
• polycomb group ring finger 4
• RING finger protein 51
• RNF51
• RNF51Polycomb group RING finger protein 4

Background

The Bmi1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMI1
• Entrez:
  • 648(Human)
  • 12151(Mouse)
  • 307151(Rat)
• Uniprot:
  • P35226()
  • P35226()
  • P35226()
  • P35226()